Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention.
نویسندگان
چکیده
The powerful strategy of "intracellular click reaction" was used to retain a chemical Ca(2+) indicator in the cytosol. Specifically, a novel clickable Ca(2+) indicator "N3-fura-2 AM" was coupled with dibenzylcyclooctyl-modified biomacromolecules via copper-free click reaction in living cells and Ca(2+) oscillation was observed for an extended period of time.
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ورودعنوان ژورنال:
- Chemical communications
دوره 49 66 شماره
صفحات -
تاریخ انتشار 2013